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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KTN1 Recombinant antibody (85681-4-RR)

    WB analysis using 85681-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85681-4-RR (KTN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KTN1 Recombinant antibody (85681-4-RR)

    IHC staining of human ovary cancer using 85681-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KTN1 Recombinant antibody (85681-4-RR)

    IHC staining of human ovary cancer using 85681-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KTN1 Recombinant antibody (85681-4-RR)

    IF Staining of HepG2 using 85681-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (85681-4-RR, Clone: 243067A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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KTN1 Recombinant antibody

KTN1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85681-4-RR
Clone No.243067A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CG-1 antigen, KIAA0004, KTN 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85681-4-RR targets KTN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KTN1 fusion protein Ag13854 相同性解析による交差性が予測される生物種
Full Name kinectin 1 (kinesin receptor)
Calculated molecular weight 1357 aa, 156 kDa
Observed molecular weight 156 kDa
GenBank accession numberBC117132
Gene Symbol KTN1
Gene ID (NCBI) 3895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86UP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

Protocols

Product Specific Protocols
WB protocol for KTN1 antibody 85681-4-RRDownload protocol
IHC protocol for KTN1 antibody 85681-4-RRDownload protocol
IF protocol for KTN1 antibody 85681-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols