Validation Data Gallery

Filter: AllWBIHCIF-PFC (Intra)

WB analysis using 17514-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 17514-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 17514-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 17514-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 17514-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 17514-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Cytokeratin 8 antibody (17514-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 17514-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 8 (17514-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 17514-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Synonyms

KRT8, CARD2, CK 8, CK8, CK-8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (26)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
Positive IHC detected in	human appendicitis tissue, human bowen disease tissue, human colon tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 12 publications below
IF	See 10 publications below

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	keratin 8
Calculated molecular weight	54 kDa
Observed molecular weight	54 kDa
GenBank accession number	NM_002273
Gene Symbol	Cytokeratin 8
Gene ID (NCBI)	3856
RRID	AB_2134726
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05787
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 8 antibody 17514-1-AP	Download protocol
IHC protocol for Cytokeratin 8 antibody 17514-1-AP	Download protocol
WB protocol for Cytokeratin 8 antibody 17514-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	IF	Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer Authors - Yifei Cheng View Article
human	WB	Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer Authors - Mengjie Li View Article
human	WB	Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. Authors - An-Dong Liu View Article
mouse	IHC	Sci Rep Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Authors - Shu Wei View Article
human	IHC	Sci Rep A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer Authors - Weiyang Yang View Article
mouse	WB,IF	iScience Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid. Authors - Jianjun Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17514-1-AP

WB analysis of C6 using 17514-1-AP

WB analysis of A549 using 17514-1-AP

WB analysis of A549 using 17514-1-AP

IHC staining of human colon using 17514-1-AP

IHC staining of human colon using 17514-1-AP

IHC staining of human appendicitis using 17514-1-AP

IHC staining of human appendicitis using 17514-1-AP

IHC staining of human bowen disease using 17514-1-AP

IHC staining of human bowen disease using 17514-1-AP

IHC staining of human bowen disease using 17514-1-AP

IHC staining of human bowen disease using 17514-1-AP

IHC staining of human prostate cancer using 17514-1-AP

IHC staining of human prostate cancer using 17514-1-AP

IF Staining of mouse testis using 17514-1-AP

FC experiment of HeLa using 17514-1-AP

Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.

A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer

Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.