Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of A431 using 10590-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 10590-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10590-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 10590-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skin using 10590-1-AP
rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 40x lens).
IHC staining of human breast cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 10590-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 6A antibody (10590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 10590-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10590-1-AP (Cytokeratin 6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10590-1-AP
Immunofluorescent analysis of Hela cells, using KRT6A antibody 10590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of A431 using 10590-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 6A (10590-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10590-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, HeLa cells, A375 cells, rat skin tissue, U-251 cells
Positive IHC detected in	human oesophagus cancer tissue, human breast cancer tissue, human skin tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HeLa cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:10000-1:50000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 12 publications below
IF	See 13 publications below

Product Information

10590-1-AP targets Cytokeratin 6A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0882 Product name: Recombinant human KRT6A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 364-564 aa of BC008807 Sequence: QVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSRKSYKH 相同性解析による交差性が予測される生物種
Full Name	keratin 6A
Calculated molecular weight	60 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC008807
Gene Symbol	Cytokeratin 6A
Gene ID (NCBI)	3853
RRID	AB_2134306
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02538
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol
IHC protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol
WB protocol for Cytokeratin 6A antibody 10590-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Cancer Cell KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy Authors - Jiacheng Guo View Article
mouse	IF	Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Authors - Fangzhou Lou View Article
		Cell Rep Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis Authors - Jiaqing Hao View Article
human,mouse	IF	Cell Rep Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer Authors - Xinyu Yang View Article
mouse	WB	Phytother Res Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization Authors - Yongling Ning View Article
human,mouse	WB,IHC,IF	J Cell Mol Med Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis. Authors - Lihua Gao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 10590-1-AP

WB analysis of U-251 using 10590-1-AP

WB analysis of MCF-7 using 10590-1-AP

WB analysis of HeLa using 10590-1-AP

WB analysis of MCF-7 using 10590-1-AP

WB analysis of A375 using 10590-1-AP

WB analysis of rat skin using 10590-1-AP

IHC staining of human oesophagus cancer using 10590-1-AP

IHC staining of human oesophagus cancer using 10590-1-AP

IHC staining of human skin using 10590-1-AP

IHC staining of human skin using 10590-1-AP

IHC staining of human lung cancer using 10590-1-AP

IHC staining of human lung cancer using 10590-1-AP

IHC staining of human breast cancer using 10590-1-AP

IHC staining of human breast cancer using 10590-1-AP

IF Staining of A431 using 10590-1-AP

IF Staining of A431 using 10590-1-AP

IF Staining of HeLa using 10590-1-AP

FC experiment of A431 using 10590-1-AP

Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.