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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10590-1-AP

Synonyms

CK 6A, CK 6D, CK6A, CK6C, CK6D, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6

Validation Data Gallery

Applications:
  • WB analysis of A431 using 10590-1-AP

    WB analysis of A431 using 10590-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat skin using 10590-1-AP

    WB analysis of rat skin using 10590-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 10590-1-AP

    WB analysis of MCF-7 using 10590-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 10590-1-AP

    WB analysis of A375 using 10590-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 10590-1-AP

    WB analysis of MCF-7 using 10590-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10590-1-AP

    WB analysis of HeLa using 10590-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-251 using 10590-1-AP

    WB analysis of U-251 using 10590-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 10590-1-AP

    IHC staining of human oesophagus cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human oesophagus cancer using 10590-1-AP

    IHC staining of human oesophagus cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human lung cancer using 10590-1-AP

    IHC staining of human lung cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human lung cancer using 10590-1-AP

    IHC staining of human lung cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human breast cancer using 10590-1-AP

    IHC staining of human breast cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human breast cancer using 10590-1-AP

    IHC staining of human breast cancer using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human skin using 10590-1-AP

    IHC staining of human skin using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human skin using 10590-1-AP

    IHC staining of human skin using 10590-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IF Staining of A431 using 10590-1-AP

    IF Staining of A431 using 10590-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 6A antibody (10590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 10590-1-AP

    IF Staining of A431 using 10590-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10590-1-AP (Cytokeratin 6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 10590-1-AP

    IF Staining of HeLa using 10590-1-AP

    Immunofluorescent analysis of Hela cells, using KRT6A antibody 10590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • FC experiment of A431 using 10590-1-AP

    FC experiment of A431 using 10590-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 6A (10590-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, HeLa cells, A375 cells, rat skin tissue, U-251 cells
Positive IHC detected inhuman oesophagus cancer tissue, human breast cancer tissue, human skin tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HeLa cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT6 Ready-To-Use IHC Kit. KRT6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10590-1-AP targets Cytokeratin 6A in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 6A fusion protein Ag0882 相同性解析による交差性が予測される生物種
Full Name keratin 6A
Calculated molecular weight 60 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC008807
Gene symbol KRT6A
Gene ID (NCBI) 3853
RRIDAB_2134306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
IHC protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
IF protocol for Cytokeratin 6A antibody 10590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
View Article

Cell Rep

Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis

Authors - Jiaqing Hao
View Article
human,mouseIF

Cell Rep

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer

Authors - Xinyu Yang
View Article
mouseWB

Phytother Res

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Authors - Yongling Ning
View Article
human,mouseWB,IHC,IF

J Cell Mol Med

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.

Authors - Lihua Gao
View Article
humanWB

J Eur Acad Dermatol Venereol

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Authors - T Yuan
View Article