• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of A431 using 16853-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of AML12 cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of AML12 using 16853-1-AP

    WB result of Cytokeratin 6A antibody (16853-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and Cytokeratin 6A knockout AML 12 cells.

  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of HeLa using 16853-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of human saliva using 16853-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    WB analysis of mouse skin using 16853-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IP experiment of HeLa using 16853-1-AP

    IP result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human bowen disease using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Cytokeratin 6A-specific Polyclonal antibody (16853-1-AP)

    IHC staining of human bowen disease using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

Cytokeratin 6A-specific Polyclonal antibody

Cytokeratin 6A-specific Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16853-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, AML12 cells, HeLa cells, human saliva, mouse skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16853-1-AP targets Cytokeratin 6A-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name keratin 6A
Calculated molecular weight 60 kDa
Observed molecular weight 56 kDa
GenBank accession numberNM_005554
Gene Symbol Cytokeratin 6A
Gene ID (NCBI) 3853
RRIDAB_2134304
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP02538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.

Protocols

Product Specific Protocols
IHC protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IP protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
WB protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...