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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KRT36 Polyclonal antibody

KRT36 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14309-1-AP

Synonyms

HA6, Hair keratin, type I Ha6, hHa6, HKA6, K36, keratin 36, Keratin, type I cuticular Ha6, KRT36, KRTHA6

Validation Data Gallery

Applications:
  • WB analysis of mouse skin using 14309-1-AP

    WB analysis of mouse skin using 14309-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 14309-1-AP

    WB analysis of mouse skin using 14309-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 14309-1-AP (KRT36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 14309-1-AP

    IHC staining of human skin cancer using 14309-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin cancer using 14309-1-AP

    IHC staining of human skin cancer using 14309-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human skin cancer using 14309-1-AP

    IHC staining of human skin cancer using 14309-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human skin cancer using 14309-1-AP

    IHC staining of human skin cancer using 14309-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14309-1-AP (KRT36 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14309-1-AP targets KRT36 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT36 fusion protein Ag5483 相同性解析による交差性が予測される生物種
Full Name keratin 36
Calculated molecular weight 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC043581
Gene symbol KRT36
Gene ID (NCBI) 8689
RRIDAB_2134018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KRT36 antibody 14309-1-APDownload protocol
IHC protocol for KRT36 antibody 14309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols