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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of HeLa using 20449-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of A375 using 20449-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of mouse skin using 20449-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of HepG2 using 20449-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of L02 using 20449-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using KRT34 Polyclonal antibody (20449-1-AP)

    WB analysis of A431 using 20449-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human lung squamous cell carcinoma using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human lung squamous cell carcinoma using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using KRT34 Polyclonal antibody (20449-1-AP)

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KRT34 Polyclonal antibody (20449-1-AP)

    IF Staining of HepG2 using 20449-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Cytokeratin 34 Polyclonal antibody

Cytokeratin 34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20449-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

keratin 34, KRT34, keratin34, Keratin-34, KRT 34

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A375 cells, A431 cells, HepG2 cells, L02 cells, mouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20449-1-AP targets Cytokeratin 34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14295

Product name: Recombinant human KRT34 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 88-436 aa of BC041070

Sequence: WFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELEKLIQERSQQQEPLLCPSYQSYFKTIEELQQKILCAKAENARLVVNIDNAKLASDDFRSKYQTEQSLRLLVESDINSIRRILDELTLCKSDLESQVESLREELICLKKNHEEEVNTLRSQLGDRLNVEVDTAPTVDLNQVLNETRSQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEAHYSSQLSQVQSLITNVESQLAEIRCDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPCNPCATTNASGNSCGPCGTSQKGCCN

相同性解析による交差性が予測される生物種
Full Name keratin 34
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC041070
Gene Symbol KRT34
Gene ID (NCBI) 3885
RRIDAB_10700000
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
IHC protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
WB protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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