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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    WB analysis of HeLa using 22230-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    WB analysis of MCF-7 using 22230-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 17 Polyclonal antibody (22230-1-AP)

    IF Staining of HeLa using 22230-1-AP

    Immunofluorescent analysis of HeLa cells using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22230-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman bowen disease, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22230-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17575

Product name: Recombinant human KRT17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of BC000159

Sequence: MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKAT

相同性解析による交差性が予測される生物種
Full Name keratin 17
Calculated molecular weight 432 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000159
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
RRIDAB_2879040
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IHC protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
WB protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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