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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRR1 Recombinant monoclonal antibody (86993-3-RR)

    WB analysis using 86993-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86993-3-RR (KRR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRR1 Recombinant monoclonal antibody (86993-3-RR)

    IF Staining of HeLa using 86993-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KRR1 antibody (86993-3-RR, Clone: 252034F1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using KRR1 Recombinant monoclonal antibody (86993-3-RR)

    IF Staining of RAW 264.7 using 86993-3-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using KRR1 antibody (86993-3-RR, Clone: 252034F1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KRR1 Recombinant monoclonal antibody

KRR1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86993-3-RR
Clone No.252034F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HIV-1 Rev-binding protein 2, HRB2, KRR-R motif-containing protein 1, Rev-interacting protein 1, RIP 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A-204 cells, PANC-1 cells, Jurkat cells, Daudi cells, RAW 264.7 cells, PC-12 cells
Positive IF/ICC detected inRAW 264.7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86993-3-RR targets KRR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3464

Product name: Recombinant human KRR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-381 aa of BC026107

Sequence: MASPSLERPEKGAGKSEFRNQKPKPENQDESELLTVPDGWKEPAFSKEDNPRGLLEESSFATLFPKYREAYLKECWPLVQKALNEHHVNATLDLIEGSMTVCTTKKTFDPYIIIRARDLIKLLARSVSFEQAVRILQDDVACDIIKIGSLVRNKERFVKRRQRLIGPKGSTLKALELLTNCYIMVQGNTVSAIGPFSGLKEVRKVALDTMKNIHPIYNIKSLMIKRELAKDSELRSQSWERFLPQFKHKNVNKRKEPKKKTVKKEYTPFPPPQPESQIDKELASGEYFLKANQKKRQKMEAIKAKQAEAISKRQEERNKAFIPPKEKPIVKPKEASTETKIDVASIKEKVKKAKNKKLGALTAEEIALKMEADEKKKKKKK

相同性解析による交差性が予測される生物種
Full Name KRR1, small subunit (SSU) processome component, homolog (yeast)
Calculated molecular weight 381 aa, 44 kDa
Observed molecular weight46 kDa
GenBank accession numberBC026107
Gene Symbol KRR1
Gene ID (NCBI) 11103
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KRR1 (also known as RIP-1) is a key assembly factor in the biogenesis of 40S precursor ribosomes (90S). As a rate-limiting node in ribosome biogenesis, its expression level reflects overall ribosome production activity.

Protocols

Product Specific Protocols
IF protocol for KRR1 antibody 86993-3-RRDownload protocol
WB protocol for KRR1 antibody 86993-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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