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KRAS-4B-specific Polyclonal antibody

KRAS-4B-specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18294-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

KRAS-4B, KRAS, pan RAS, GTPase KRas, EC:3.6.5.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IHC detected inhuman pancreas tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18294-1-AP targets KRAS-4B-specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
GenBank accession numberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, 4A and 4B. The antibody is specific to KRAS, can't recognize NRAS and HRAS.

Protocols

Product Specific Protocols
IHC protocol for KRAS-4B-specific antibody 18294-1-APDownload protocol
IF protocol for KRAS-4B-specific antibody 18294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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