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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of A549 using 12063-1-AP (same clone as 12063-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat kidney using 12063-1-AP (same clone as 12063-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat liver using 12063-1-AP (same clone as 12063-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human brain using 12063-1-AP (same clone as 12063-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:10000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KRAS Polyclonal antibody, PBS Only

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12063-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12063-1-PBS targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAS fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.