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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using KPNA6 Polyclonal antibody (12366-2-AP)

    WB analysis of BxPC-3 using 12366-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KPNA6 Polyclonal antibody (12366-2-AP)

    WB analysis of HeLa using 12366-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KPNA6 Polyclonal antibody (12366-2-AP)

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KPNA6 Polyclonal antibody (12366-2-AP)

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KPNA6 Polyclonal antibody (12366-2-AP)

    IHC staining of human prostate cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KPNA6 Polyclonal antibody (12366-2-AP)

    IHC staining of human prostate cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KPNA6 Polyclonal antibody (12366-2-AP)

    IHC staining of human prostate cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12366-2-AP (KPNA6 antibody) at dilution of 1:50 (under 10x lens).

KPNA6 Polyclonal antibody

KPNA6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12366-2-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12366-2-AP targets KPNA6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3040

Product name: Recombinant human KPNA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 185-536 aa of BC020520

Sequence: EQAVWALGNIAGDSSVCRDYVLNCSILNPLLTLLTKSTRLTMTRNAVWALSNLCRGKNPPPEFAKVSPCLPVLSRLLFSSDSDLLADACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHNDYKVASPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIRKEACWTISNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVSLGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEGKRSGSGVNPYCGLIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEDDDSSLAPQVDETQQQFIFQQPEAPMEGFQL

相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 6 (importin alpha 7)
Calculated molecular weight 536 aa, 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC020520
Gene Symbol KPNA6
Gene ID (NCBI) 23633
RRIDAB_2281067
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for KPNA6 antibody 12366-2-APDownload protocol
WB protocol for KPNA6 antibody 12366-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco
View Article
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
View Article
humanWB

Viruses

Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2

Authors - Anna Nakanishi
View Article
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