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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPNA4 Recombinant antibody

KPNA4 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230098H1

Cat no : 82898-2-RR

Synonyms

Importin alpha Q1, Importin subunit alpha 4, IPOA3, Karyopherin subunit alpha 4, KPNA4, QIP1, SRP3

Validation Data Gallery

Applications:
  • WB analysis using 82898-2-RR

    WB analysis using 82898-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 82898-2-RR

    IF Staining of U2OS using 82898-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

82898-2-RR targets KPNA4 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated molecular weight 521 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC034493
Gene symbol KPNA4
Gene ID (NCBI) 3840
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Protocols

Product Specific Protocols
WB protocol for KPNA4 antibody 82898-2-RRDownload protocol
IF protocol for KPNA4 antibody 82898-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols