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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G9C1

Cat no : 66870-1-Ig

Synonyms

Importin subunit alpha 2, IPOA1, Karyopherin subunit alpha 2, KPNA2, QIP2, RAG cohort protein 1, RCH1, SRP1, SRP1 alpha, SRP1alpha

Validation Data Gallery

Applications:
  • WB analysis using 66870-1-Ig

    WB analysis using 66870-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66870-1-Ig

    IF Staining of MCF-7 using 66870-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA2 Ready-To-Use IHC Kit. KPNA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66870-1-Ig targets KPNA2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KPNA2 fusion protein Ag28386 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Calculated molecular weight 57.8 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC005978
Gene symbol KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

Protocols

Product Specific Protocols
WB protocol for KPNA2 antibody 66870-1-IgDownload protocol
IF protocol for KPNA2 antibody 66870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols