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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using KLRG1 Recombinant antibody (84785-5-RR)

    IF Staining of Jurkat using 84785-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using KLRG1 antibody (84785-5-RR, Clone: 242327A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of NK-92 cells using KLRG1 Recombinant antibody (84785-5-RR)

    IF Staining of NK-92 using 84785-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using KLRG1 antibody (84785-5-RR, Clone: 242327A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KLRG1 Recombinant monoclonal antibody

KLRG1 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84785-5-RR
Clone No.242327A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

MAFA, Killer cell lectin-like receptor subfamily G member 1, ITIM-containing receptor MAFA-L, C-type lectin domain family 15 member A, CLEC15A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNK-92 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84785-5-RR targets KLRG1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily G, member 1
Calculated molecular weight22 kDa
GenBank accession numberNM_005810.4
Gene Symbol KLRG1
Gene ID (NCBI) 10219
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96E93-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

Protocols

Product Specific Protocols
IF protocol for KLRG1 antibody 84785-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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