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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis using 10974-1-AP (same clone as 10974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis of human peripheral blood leukocyte using 10974-1-AP (same clone as 10974-1-PBS)

    Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis of Raji using 10974-1-AP (same clone as 10974-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KLRG1 Polyclonal antibody (10974-1-AP)

    IHC staining of human breast cancer using 10974-1-AP (same clone as 10974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KLRG1 Polyclonal antibody (10974-1-AP)

    IHC staining of human breast cancer using 10974-1-AP (same clone as 10974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

KLRG1 Polyclonal antibody, PBS Only

KLRG1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 10974-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MAFA 2F1, MAFA, Killer cell lectin-like receptor subfamily G member 1, ITIM-containing receptor MAFA-L, C-type lectin domain family 15 member A

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10974-1-PBS targets KLRG1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1407

Product name: Recombinant human KLRG1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-195 aa of BC012621

Sequence: MTDSVIYSMLELPTATQAQNDYGPQQKSSSSRPSCSCLVAIALGLLTAVLLSVLLYQWILCQGSNYSTCASCPSCPDRWMKYGNHCYYFSVEEKDWNSSLEFCLARDSHLLVITDNQEMSLLQVFLSEAFCWIGLRNNSGWRWEDGSPLNFSRISSNSFVQTCGAINKNGLQASSCEVPLHWVCKKCPFADQALF

相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily G, member 1
Calculated molecular weight 22 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC012621
Gene Symbol KLRG1
Gene ID (NCBI) 10219
RRIDAB_2234383
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96E93
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role in NK- and T-cell function (PMID: 19009530; 20373518). Because of the glycosylation and disulfide bonds of the KLRG1 proteins, the observed molecular weights are typically 30-38 kDa (PMID: 17404256).

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