Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-468 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12264-1-AP targets Kallikrein 6 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2930 Product name: Recombinant human KLK6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC015525 Sequence: MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAK 相同性解析による交差性が予測される生物種 |
| Full Name | kallikrein-related peptidase 6 |
| Calculated molecular weight | 244 aa, 28 kDa |
| GenBank accession number | BC015525 |
| Gene Symbol | KLK6 |
| Gene ID (NCBI) | 5653 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92876 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kallikrein 6 (KLK6, also named neurosin) is a secreted serine protease encoded by the KLK6 gene on human chromosome 19q13.41. It is highly expressed in the central nervous system and epithelial tissues. KLK6 participates in extracellular matrix remodeling, regulates amyloid and α-synuclein metabolism, and modulates inflammatory responses. Dysregulated KLK6 facilitates tumor invasion and metastasis in multiple malignancies. It is regarded as a valuable diagnostic and prognostic biomarker for cancers and neurodegenerative diseases, representing a promising therapeutic target. (PMID: 35883559)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Kallikrein 6 antibody 12264-1-AP | Download protocol |
| WB protocol for Kallikrein 6 antibody 12264-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |