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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kallikrein 11 Polyclonal antibody

Kallikrein 11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12698-1-AP

Synonyms

Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 12698-1-AP

    WB analysis of MCF-7 using 12698-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12698-1-AP

    WB analysis of HeLa using 12698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 12698-1-AP

    WB analysis of Y79 using 12698-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 12698-1-AP

    WB analysis of PC-3 using 12698-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12698-1-AP

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human prostate cancer using 12698-1-AP

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human brain using 12698-1-AP

    IHC staining of human brain using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human brain using 12698-1-AP

    IHC staining of human brain using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human prostate cancer using 12698-1-AP

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IF Staining of PC-3 using 12698-1-AP

    IF Staining of PC-3 using 12698-1-AP

    Immunofluorescent analysis of PC-3 cells using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, PC-3 cells, Y79 cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12698-1-AP targets Kallikrein 11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kallikrein 11 fusion protein Ag3427 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 11
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC022068
Gene symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2877876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
IHC protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
IF protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols