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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL34 Polyclonal antibody (21981-1-AP)

    WB analysis using 21981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21981-1-AP (KLHL34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using KLHL34 Polyclonal antibody (21981-1-AP)

    IHC staining of human small intestine using 21981-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using KLHL34 Polyclonal antibody (21981-1-AP)

    IHC staining of human small intestine using 21981-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 40x lens).

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KLHL34 Polyclonal antibody

KLHL34 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21981-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Synonyms

kelch like 34 (Drosophila), Kelch like protein 34, KLHL34

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21981-1-AP targets KLHL34 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL34 fusion protein Ag16001 相同性解析による交差性が予測される生物種
Full Name kelch-like 34 (Drosophila)
Calculated molecular weight 644 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC109031
Gene Symbol KLHL34
Gene ID (NCBI) 257240
RRIDAB_2878959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLHL34 is a member of the Kelch-like (KLHL) gene family, which encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain and 5-6 Kelch motifs. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. The molecular mass of KLHL34 is 71 kDa.

Protocols

Product Specific Protocols
WB protocol for KLHL34 antibody 21981-1-APDownload protocol
IHC protocol for KLHL34 antibody 21981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols