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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL32 Polyclonal antibody (28621-1-AP)

    WB analysis using 28621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28621-1-AP (KLHL32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KLHL32 Polyclonal antibody (28621-1-AP)

    IF Staining of U2OS using 28621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KLHL32 antibody (28621-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KLHL32 Polyclonal antibody

KLHL32 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28621-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RP1 39B17.1, KIAA1900, Kelch-like protein 32, Kelch like protein 32, BTB and kelch domain-containing protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, PC-3 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28621-1-AP targets KLHL32 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL32 fusion protein Ag29003 相同性解析による交差性が予測される生物種
Full Name kelch-like 32 (Drosophila)
Calculated molecular weight 470 aa, 53 kDa
Observed molecular weight70 kDa
GenBank accession numberBC071627
Gene Symbol KLHL32
Gene ID (NCBI) 114792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLHL32 (Kelch-like protein 32), also known BKLHD5, which is located in the cytoplasm. The expression level is high in the central nervous system, especially in astrocytes. In addition, it is also expressed in the motor cilia of fallopian tube. But the overall expression abundance is low. KLHL32 is mainly involved in the intracellular ubiquitination process. Ubiquitin is an important cellular regulatory mechanism, through which protein can be labeled to degrade or change its function. KLHL32 interacts with other protein through its BTB domain and Kelch repeat domain to help regulate this process. It is also a prognostic marker in glioblastoma and renal clear cell carcinoma. The molecular weight of KLHL32 is 70 kDa.

Protocols

Product Specific Protocols
WB protocol for KLHL32 antibody 28621-1-APDownload protocol
IF protocol for KLHL32 antibody 28621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols