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  • Featured Product
  • KD/KO Validated

KLHL31 Polyclonal antibody

KLHL31 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24781-1-AP

Synonyms

BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31

Validation Data Gallery

Applications:
  • WB analysis using 24781-1-AP

    WB analysis using 24781-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse embryo using 24781-1-AP

    WB analysis of mouse embryo using 24781-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 24781-1-AP

    WB analysis of Jurkat using 24781-1-AP

    WB result of KLHL31 antibody (24781-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLHL31 transfected Jurkat cells.

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24781-1-AP

    IHC staining of human colon cancer using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24781-1-AP (KLHL31 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 24781-1-AP

    IHC staining of human colon cancer using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24781-1-AP (KLHL31 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24781-1-AP

    IF Staining of HepG2 using 24781-1-AP

    Immunofluorescent analysis of HepG2 cells using 24781-1-AP (KLHL31 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse embryo tissue, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

24781-1-AP targets KLHL31 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL31 fusion protein Ag20462 相同性解析による交差性が予測される生物種
Full Name kelch-like 31 (Drosophila)
Calculated molecular weight 634 aa, 70 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC137267
Gene symbol KLHL31
Gene ID (NCBI) 401265
RRIDAB_2879718
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLHL31 also named as BKLHD6, KBTBD1,or Kelch like protein 31 is a 634 amino acid protein, which contains one 1 BACK domain, one BTB domain and six Kelch repeats. KLHL31 as a transcription repressor in MAPK/JNK signaling pathway to regulate cellular functions. KLHL31 is strongly expressed in skeletal muscle and weakly in heart.

Protocols

Product Specific Protocols
WB protocol for KLHL31 antibody 24781-1-APDownload protocol
IHC protocol for KLHL31 antibody 24781-1-APDownload protocol
IF protocol for KLHL31 antibody 24781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols