Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HT-29 cells, HepG2 cells, NCCIT cells, MOLT-4 cells, Raji cells, Ramos cells, Daudi cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67774-1-Ig targets KLHL25 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27037 Product name: Recombinant human KLHL25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 162-260 aa of BC028100 Sequence: RRLYEFSWRMCLVHFETVRQSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLALLPSDCLQEAVSSEALLMADE 相同性解析による交差性が予測される生物種 |
| Full Name | kelch-like 25 (Drosophila) |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC028100 |
| Gene Symbol | KLHL25 |
| Gene ID (NCBI) | 64410 |
| RRID | AB_2918539 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H0H3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KLHL25 antibody 67774-1-Ig | Download protocol |
| WB protocol for KLHL25 antibody 67774-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
