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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using KLHL13 Polyclonal antibody (15612-1-AP)

    WB analysis of Jurkat using 15612-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15612-1-AP (KLHL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KLHL13 Polyclonal antibody (15612-1-AP)

    WB analysis of HeLa using 15612-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15612-1-AP (KLHL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using KLHL13 Polyclonal antibody (15612-1-AP)

    IHC staining of human lung using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using KLHL13 Polyclonal antibody (15612-1-AP)

    IHC staining of mouse skeletal muscle using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using KLHL13 Polyclonal antibody (15612-1-AP)

    IHC staining of mouse skeletal muscle using 15612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15612-1-AP (KLHL13 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KLHL13 Polyclonal antibody

KLHL13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15612-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309, KLHL13

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15612-1-AP targets KLHL13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8003

Product name: Recombinant human KLHL13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 487-655 aa of BC008729

Sequence: GGVMYISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYYSPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSPSRESPLSAP

相同性解析による交差性が予測される生物種
Full Name kelch-like 13 (Drosophila)
Calculated molecular weight 74 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008729
Gene Symbol KLHL13
Gene ID (NCBI) 90293
RRIDAB_2130739
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for KLHL13 antibody 15612-1-APDownload protocol
WB protocol for KLHL13 antibody 15612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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