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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    WB analysis of PC-3 using 14883-1-AP (same clone as 14883-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    WB analysis of PC-3 using 14883-1-AP (same clone as 14883-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL12 Polyclonal antibody (14883-1-AP)

    IHC staining of human testis using 14883-1-AP (same clone as 14883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KLHL12 Polyclonal antibody (14883-1-AP)

    IHC staining of human testis using 14883-1-AP (same clone as 14883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14883-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using KLHL12 Polyclonal antibody (14883-1-AP)

    IF Staining of PC-3 using 14883-1-AP (same clone as 14883-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using KLHL12 antibody (14883-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 14883-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KLHL12 Polyclonal antibody, PBS Only

KLHL12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14883-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C3IP1, CUL3 interacting protein 1, CUL3-interacting protein 1, DKIR, DKIR homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14883-1-PBS targets KLHL12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6658

Product name: Recombinant human KLHL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 217-568 aa of BC004175

Sequence: NLLQYVRMPLLTPRYITDVIDAEPFIRCSLQCRDLVDEAKKFHLRPELRSQMQGPRTRARLGANEVLLVVGGFGSQQSPIDVVEKYDPKTQEWSFLPSITRKRRYVASVSLHDRIYVIGGYDGRSRLSSVECLDYTADEDGVWYSVAPMNVRRGLAGATTLGDMIYVSGGFDGSRRHTSMERYDPNIDQWSMLGDMQTAREGAGLVVASGVIYCLGGYDGLNILNSVEKYDPHTGHWTNVTPMATKRSGAGVALLNDHIYVVGGFDGTAHLSSVEAYNIRTDSWTTVTSMTTPRCYVGATVLRGRLYAIAGYDGNSLLSSIECYDPIIDSWEVVTSMGTQRCDAGVCVLREK

相同性解析による交差性が予測される生物種
Full Name kelch-like 12 (Drosophila)
Calculated molecular weight 63 kDa
Observed molecular weight 53-56 kDa, 63-67 kDa
GenBank accession numberBC004175
Gene Symbol KLHL12
Gene ID (NCBI) 59349
RRIDAB_10644282
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53G59
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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