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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL10 Polyclonal antibody (25591-1-AP)

    WB analysis using 25591-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25591-1-AP (KLHL10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using KLHL10 Polyclonal antibody (25591-1-AP)

    WB analysis of mouse testis using 25591-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25591-1-AP (KLHL10 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of rat testis tissue using KLHL10 Polyclonal antibody (25591-1-AP)

    WB analysis of rat testis using 25591-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 25591-1-AP (KLHL10 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

KLHL10 Polyclonal antibody

KLHL10 Polyclonal Antibody for WB, ELISA
Cat No. 25591-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Kelch-like protein 10, Kelch like protein 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25591-1-AP targets KLHL10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL10 fusion protein Ag22368 相同性解析による交差性が予測される生物種
Full Name kelch-like 10 (Drosophila)
Calculated molecular weight 608 aa, 69 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC067753
Gene Symbol KLHL10
Gene ID (NCBI) 317719
RRIDAB_2880146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6JEL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL10 antibody 25591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols