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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHDC5 Polyclonal antibody (24847-1-AP)

    WB analysis of HeLa using 24847-1-AP (same clone as 24847-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24847-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    WB analysis of mouse brain using 24847-1-AP (same clone as 24847-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    IHC staining of human kidney using 24847-1-AP (same clone as 24847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24847-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLHDC5 Polyclonal antibody (24847-1-AP)

    IHC staining of human kidney using 24847-1-AP (same clone as 24847-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24847-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLHDC5 Polyclonal antibody (24847-1-AP)

    IF Staining of HeLa using 24847-1-AP (same clone as 24847-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24847-1-PBS in a different storage buffer formulation.

KLHDC5 Polyclonal antibody, PBS Only

KLHDC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24847-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ctb9, Cullin-3-binding protein 9, kelch domain containing 5, Kelch domain-containing protein 5, Kelch-like protein 42

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24847-1-PBS targets KLHDC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20448

Product name: Recombinant human KLHDC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-505 aa of BC137116

Sequence: FHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET

相同性解析による交差性が予測される生物種
Full Name kelch domain containing 5
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC137116
Gene Symbol KLHDC5
Gene ID (NCBI) 57542
RRIDAB_2879754
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

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