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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLF5 Recombinant antibody (83205-4-RR)

    IF Staining of HeLa using 83205-4-RR (same clone as 83205-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using KLF5 Recombinant antibody (83205-4-RR)

    FC experiment of A549 using 83205-4-RR (same clone as 83205-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using KLF5 Recombinant antibody (83205-4-RR)

    FC experiment of HT-29 using 83205-4-RR (same clone as 83205-4-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83205-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83205-4-RR

    Affinity and Kinetic Characterization of 83205-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

KLF5 Recombinant monoclonal antibody, PBS Only

KLF5 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83205-4-PBS
Clone No.230509G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83205-4-PBS targets KLF5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15267

Product name: Recombinant human KLF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC042131

Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN

相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13887
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

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