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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis using 21017-1-AP (same clone as 21017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of PC-3 using 21017-1-AP (same clone as 21017-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of HT-1080 using 21017-1-AP (same clone as 21017-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of A549 using 21017-1-AP (same clone as 21017-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of mouse small intestine using 21017-1-AP (same clone as 21017-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of A549 using 21017-1-AP (same clone as 21017-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of mouse colon using 21017-1-AP (same clone as 21017-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using KLF5 Polyclonal antibody (21017-1-AP)

    WB analysis of human testis using 21017-1-AP (same clone as 21017-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using KLF5 Polyclonal antibody (21017-1-AP)

    IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of mouse embryo using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using KLF5 Polyclonal antibody (21017-1-AP)

    IHC staining of mouse embryo using 21017-1-AP (same clone as 21017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLF5 Polyclonal antibody (21017-1-AP)

    IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (21017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21017-1-PBS targets KLF5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15267 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_10696447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13887
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).