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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis using 11880-1-AP (same clone as 11880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis using 11880-1-AP (same clone as 11880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis using 11880-1-AP (same clone as 11880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis using 11880-1-AP (same clone as 11880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis using 11880-1-AP (same clone as 11880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using KLF4 Polyclonal antibody (11880-1-AP)

    WB analysis of NCCIT using 11880-1-AP (same clone as 11880-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 11880-1-AP (KLF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using KLF4 Polyclonal antibody (11880-1-AP)

    IP experiment of HeLa using 11880-1-AP (same clone as 11880-1-PBS)

    IP result of anti-KLF4 (IP:11880-1-AP, 4ug; Detection:11880-1-AP 1:8000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of mouse embryo using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsil tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human tonsil using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsil tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human tonsil using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human spleen using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human spleen using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human colon using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of human colon using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 11880-1-AP (KLF4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using KLF4 Polyclonal antibody (11880-1-AP)

    IHC staining of mouse embryo using 11880-1-AP (same clone as 11880-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11880-1-AP (KLF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human embronic stem cells using KLF4 Polyclonal antibody (11880-1-AP)

    IF Staining of human embronic stem cells using 11880-1-AP (same clone as 11880-1-PBS)

    Confocal immunofluorescent analysis of human embronic stem cells with 11880-1-AP at dilution of 1:50. The PE shows staining with 11880-1-AP/PE. The DAPI shows nuclear staining by DAPI. This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KLF4 Polyclonal antibody (11880-1-AP)

    FC experiment of HeLa using 11880-1-AP (same clone as 11880-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KLF4 (11880-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11880-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KLF4 Polyclonal antibody, PBS Only

KLF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11880-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11880-1-PBS targets KLF4 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2480

Product name: Recombinant human KLF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-504 aa of BC030811

Sequence: IPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPLELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF

相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 4 (gut)
Calculated molecular weight 504 aa, 54 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC030811
Gene Symbol KLF4
Gene ID (NCBI) 9314
RRIDAB_10640807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43474
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4 (PMID: 15561714), with MW range from 50 to 60 kDa,and major band is 55 kDa. KLF4 protein can be ubiquitin modified.

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