Validation Data Gallery
Tested Applications
| Positive WB detected in | bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31170-1-AP targets KLF2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33962 Product name: Recombinant human KLF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-225 aa of NM_016270 Sequence: GLVSELLRPELDAPLGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGLTRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLHYAPPAPPAFGLFDDA 相同性解析による交差性が予測される生物種 |
| Full Name | Kruppel-like factor 2 (lung) |
| Calculated molecular weight | 37KD |
| Observed molecular weight | 37-40 kDa |
| GenBank accession number | NM_016270 |
| Gene Symbol | KLF2 |
| Gene ID (NCBI) | 10365 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y5W3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KLF2 (Kruppel-like Factor 2) belongs to zinc finger transcription factors, and it is implicated in a variety of biochemical processes, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis (PMID: 17904406). KLF2 was identified as a negative regulator of T cell activation and adipogenesis. KLF2 also acts as a novel regulator of endothelial proinflammatory activation, inhibiting the inflammatory cytokine-mediated induction of adhesion molecule expression and immune cell adhesion (PMID:15718498). KLF2-null mice exhibit abnormal blood vessel formation, resulting in embryonic haemorrhage and death (PMID: 32132543). KLF2 can be detected 60 kDa by post-translationally modified (PMID: 36466816).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF2 antibody 31170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |