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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLF10 Polyclonal antibody (29709-1-AP)

    WB analysis using 29709-1-AP (same clone as 29709-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29709-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF10 Polyclonal antibody (29709-1-AP)

    WB analysis using 29709-1-AP (same clone as 29709-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using KLF10 Polyclonal antibody (29709-1-AP)

    IHC staining of mouse pancreas using 29709-1-AP (same clone as 29709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using KLF10 Polyclonal antibody (29709-1-AP)

    IHC staining of mouse pancreas using 29709-1-AP (same clone as 29709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29709-1-PBS in a different storage buffer formulation.

KLF10 Polyclonal antibody, PBS Only

KLF10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29709-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29709-1-PBS targets KLF10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31772

Product name: Recombinant human KLF10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC011538

Sequence: MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIAAPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHLNVEAARKNIPCAAVSPNRSKCERNTVADVDEKA

相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 10
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight52-75 kDa
GenBank accession numberBC011538
Gene Symbol KLF10
Gene ID (NCBI) 7071
RRIDAB_2918341
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13118
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. KLF10 plays a role in the response to TGF beta (through the TGF beta/Smad signaling pathway) and estrogen. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer. KLF10 can be detected 51-52 kDa, 60-75 kDa (PMID: 21262377, PMID: 30026232).

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