Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
11881-1-AP targets KLF10 in WB, IP, IF, IHC, Chip, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2482 Product name: Recombinant human KLF10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-469 aa of BC011538 Sequence: MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIAAPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHLNVEAARKNIPCAAVSPNRSKCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQQKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIRSHICSHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFACPMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ 相同性解析による交差性が予測される生物種 |
| Full Name | Kruppel-like factor 10 |
| Calculated molecular weight | 469 aa, 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC011538 |
| Gene Symbol | KLF10 |
| Gene ID (NCBI) | 7071 |
| RRID | AB_2877801 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13118 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KLF10 antibody 11881-1-AP | Download protocol |
| IP protocol for KLF10 antibody 11881-1-AP | Download protocol |
| WB protocol for KLF10 antibody 11881-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Polymorphism rs7278468 is associated with Age-related cataract through decreasing transcriptional activity of the CRYAA promoter. | ||
J Mol Cell Cardiol Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation. | ||
Acta Histochem KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression. | ||
Cell Death Discov Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis | ||
Cell Signal Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis |
