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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLC4 Polyclonal antibody (16493-1-AP)

    WB analysis using 16493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16493-1-AP (KLC4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of human kidney using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of human small intestine using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of mouse stomach using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of human pancreas cancer using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of human placenta using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of mouse liver using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of mouse pancreas using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of rat liver using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of rat pancreas using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of rat stomach using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of human pancreas cancer using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of mouse stomach using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using KLC4 Polyclonal antibody (16493-1-AP)

    IHC staining of rat stomach using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KLC4 Polyclonal antibody

KLC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16493-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Kinesin-like protein 8, KLC 4, KNSL8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman small intestine tissue, human placenta tissue, human pancreas cancer tissue, human kidney tissue, rat pancreas tissue, rat liver tissue, mouse liver tissue, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16493-1-AP targets KLC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9621

Product name: Recombinant human KLC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC012357

Sequence: MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVLQSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVSIPCPPHPTPRTPHHCCFGLS

相同性解析による交差性が予測される生物種
Full Name kinesin light chain 4
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight69 kDa
GenBank accession numberBC012357
Gene Symbol KLC4
Gene ID (NCBI) 89953
RRIDAB_2923563
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for KLC4 antibody 16493-1-APDownload protocol
WB protocol for KLC4 antibody 16493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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