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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLC4 Polyclonal antibody

KLC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16493-1-AP

Synonyms

kinesin light chain 4, Kinesin like protein 8, KLC 4, KLC4, KNSL8

Validation Data Gallery

Applications:
  • WB analysis using 16493-1-AP

    WB analysis using 16493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16493-1-AP (KLC4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16493-1-AP

    IHC staining of human kidney using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 16493-1-AP

    IHC staining of human small intestine using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16493-1-AP targets KLC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLC4 fusion protein Ag9621 相同性解析による交差性が予測される生物種
Full Name kinesin light chain 4
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight69 kDa
GenBank accession numberBC012357
Gene symbol KLC4
Gene ID (NCBI) 89953
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLC4 antibody 16493-1-APDownload protocol
IHC protocol for KLC4 antibody 16493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols