c-Kit/CD117 Polyclonal antibody
c-Kit/CD117 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18696-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Mo7e cells |
| Positive IHC detected in | stromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 8 publications below |
| IF | See 7 publications below |
Product Information
18696-1-AP targets c-Kit/CD117 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, sheep, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Calculated molecular weight | 110 kDa |
| Observed molecular weight | 140-160 kDa |
| GenBank accession number | BC071593 |
| Gene symbol | KIT |
| Gene ID (NCBI) | 3815 |
| RRID | AB_2249558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
| IHC protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
| IF protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
| FC protocol for c-Kit/CD117 antibody 18696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mech Ageing Dev Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy | ||
Front Cell Dev Biol Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis. | ||
Front Immunol Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment. | ||
J Exp Clin Cancer Res Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells. | ||
Andrology Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes | ||
Tumour Biol Orai1 mediates tumor-promoting store-operated Ca(2+) entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway. |
