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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of SH-SY5Y using 15505-1-AP (same clone as 15505-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of HeLa using 15505-1-AP (same clone as 15505-1-PBS)

    Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of HepG2 using 15505-1-AP (same clone as 15505-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

KISS1R-Specific Polyclonal antibody, PBS Only

KISS1R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15505-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15505-1-PBS targets KISS1R-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name KISS1 receptor
Calculated molecular weight 43 kDa
Observed molecular weight75 kDa, 43-45 kDa
GenBank accession numberNM_032551
Gene Symbol KISS1R
Gene ID (NCBI) 84634
RRIDAB_10640578
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969F8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

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