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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KISS1 Polyclonal antibody (18375-1-AP)

    WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using KISS1 Polyclonal antibody (18375-1-AP)

    IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using KISS1 Polyclonal antibody (18375-1-AP)

    IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KISS1 Polyclonal antibody (18375-1-AP)

    IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KISS1 Polyclonal antibody (18375-1-AP)

    IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KISS1 Polyclonal antibody (18375-1-AP)

    IHC staining of human lung cancer using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using KISS1 Polyclonal antibody, PBS Only (18375-1-PBS)

    IF Staining of NIH/3T3 using 18375-1-PBS

    Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

KISS1 Polyclonal antibody, PBS Only

KISS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18375-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18375-1-PBS targets KISS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13240

Product name: Recombinant human KISS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-138 aa of BC022819

Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG

相同性解析による交差性が予測される生物種
Full Name KiSS-1 metastasis-suppressor
Calculated molecular weight 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC022819
Gene Symbol KISS1
Gene ID (NCBI) 3814
RRIDAB_11182705
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15726
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

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