Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cells, mouse brain tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22232-1-AP targets KIRREL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17578 Product name: Recombinant human KIRREL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 607-757 aa of BC109192 Sequence: NVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV 相同性解析による交差性が予測される生物種 |
| Full Name | kin of IRRE like (Drosophila) |
| Calculated molecular weight | 757 aa, 84 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC109192 |
| Gene Symbol | KIRREL |
| Gene ID (NCBI) | 55243 |
| RRID | AB_3085689 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96J84 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kin of IRRE protein (KIRREL), also known as NEPH1, is a member of a podocin-binding protein family. KIRREL is a component of the podocyte slit diaphragm, which serves as the structural framework of the glomerular filtration barrier. KIRREL has three isoforms with molecular weights of 85 kDa, 83 kDa, and 72 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIRREL antibody 22232-1-AP | Download protocol |
| IF protocol for KIRREL antibody 22232-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
