• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HL-60 cells using KIR2DS4/CD158i Polyclonal antibody (21152-1-AP)

    WB analysis of HL-60 using 21152-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using KIR2DS4/CD158i Polyclonal antibody (21152-1-AP)

    WB analysis of Jurkat using 21152-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using KIR2DS4/CD158i Polyclonal antibody (21152-1-AP)

    WB analysis of HL-60 using 21152-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIR2DS4/CD158i Polyclonal antibody (21152-1-AP)

    IF Staining of HepG2 using 21152-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIR2DS4/CD158i antibody 21152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KIR2DS4/CD158i Polyclonal antibody

KIR2DS4/CD158i Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21152-1-AP targets KIR2DS4/CD158i in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15711

Product name: Recombinant human KIR2DS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-304 aa of BC096694

Sequence: PQEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA

相同性解析による交差性が予測される生物種
Full Name killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
Calculated molecular weight 304 aa, 34 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC096694
Gene Symbol KIR2DS4
Gene ID (NCBI) 3809
RRIDAB_10732956
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43632
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.

Protocols

Product Specific Protocols
IF protocol for KIR2DS4/CD158i antibody 21152-1-APDownload protocol
WB protocol for KIR2DS4/CD158i antibody 21152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...