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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIFC1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-20 ng/mL

Cat no : MP02316-1

Print datasheet

Synonyms

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02316-1, Human KIFC1 Recombinant Matched Antibody Pair - PBS only. 85981-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14492. 85981-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

    Sandwich ELISA standard curve of MP02316-1

    Sandwich ELISA standard curve of MP02316-1, Human KIFC1 Recombinant Matched Antibody Pair - PBS only. 85981-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14492. 85981-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • The mean KIFC1 concentration was determined to be 40.55 ng/mL in HuH-7 cell extract based on a 1.40 mg/mL extract load and 31.65 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

    Sample test of MP02316-1

    The mean KIFC1 concentration was determined to be 40.55 ng/mL in HuH-7 cell extract based on a 1.40 mg/mL extract load and 31.65 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02316-1 targets KIFC1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: KIFC1 Recombinant antibody, PBS Only (Capture) 85981-4-PBS (250588B8). 100 μg. Concentration 1 mg/ml.

Detection antibody: KIFC1 Recombinant antibody, PBS Only (Detector) 85981-3-PBS (250588E4). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag14492

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 85981-4-PBS
Tested Reactivity human
Immunogen KIFC1 fusion protein Ag14492
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member C1
Synonyms HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2
GenBank accession numberBC000712
Gene symbol KIFC1
UniprotQ9BW19
Gene ID (NCBI) 3833
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 85981-3-PBS
Tested Reactivity human
Immunogen KIFC1 fusion protein Ag14492
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member C1
Synonyms HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2
GenBank accession numberBC000712
Gene symbol KIFC1
UniprotQ9BW19
Gene ID (NCBI) 3833
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.