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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIFC1 Recombinant antibody (85981-1-RR)

    WB analysis of HeLa using 85981-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using KIFC1 Recombinant antibody (85981-1-RR)

    WB analysis of NIH/3T3 using 85981-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of mouse testis using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of mouse testis using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of human tonsillitis using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of human tonsillitis using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of human placenta using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KIFC1 Recombinant antibody (85981-1-RR)

    IHC staining of human placenta using 85981-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KIFC1 Recombinant antibody (85981-1-RR)

    IF Staining of HeLa using 85981-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIFC1 antibody (85981-1-RR, Clone: 250588F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85981-1-RR

    Affinity and Kinetic Characterization of 85981-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85981-1-RR against Human KIFC1 were performed. The affinity constant is below 1 pM.

KIFC1 Recombinant monoclonal antibody

KIFC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85981-1-RR
Clone No.250588F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85981-1-RR targets KIFC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14492

Product name: Recombinant human KIFC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 382-725 aa of BC000712

Sequence: GLLLFPSGPGGPSDPPTRLSLSRSDERRGTLSGAPAPPPRHDFSFDRVFPPGSGQDEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTMEGGPGGDPQLEGLIPRALRHLFSVAQELSGQGWTYSFVASYVEIYNETVRDLLATGTRKGQGGECEIRRAGPGSEELTVTNARYVPVSCEKEVDALLHLARQNRAVARTAQNERSSRSHSVFQLQISGEHSSRGLQCGAPLSLVDLAGSERLDPGLALGPGERERLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANRK

相同性解析による交差性が予測される生物種
Full Name kinesin family member C1
Calculated molecular weight 673 aa, 74 kDa
Observed molecular weight74 kDa
GenBank accession numberBC000712
Gene Symbol KIFC1
Gene ID (NCBI) 3833
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BW19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport (PMID: 12826589, PMID: 15236353, PMID: 23543461).

Protocols

Product Specific Protocols
IF protocol for KIFC1 antibody 85981-1-RRDownload protocol
IHC protocol for KIFC1 antibody 85981-1-RRDownload protocol
WB protocol for KIFC1 antibody 85981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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