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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

KIF5B Recombinant antibody, PBS Only

KIF5B Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B18

Cat no : 82485-1-PBS

Synonyms

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 82485-1-RR (same clone as 82485-1-PBS)

    WB analysis of HeLa using 82485-1-RR (same clone as 82485-1-PBS)

    WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

  • WB analysis using 82485-1-RR (same clone as 82485-1-PBS)

    WB analysis using 82485-1-RR (same clone as 82485-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

82485-1-PBS targets KIF5B in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIF5B fusion protein Ag16250 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5B
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC126279
Gene symbol KIF5B
Gene ID (NCBI) 3799
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).