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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb™ KIF5A Multi-Recombinant antibody (RMX00017)

    WB analysis using RMX00017

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00017 (KIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of U-87 MG cells using Multi-rAb™ KIF5A Multi-Recombinant antibody (RMX00017)

    IP experiment of U-87 MG using RMX00017

    IP result of anti-KIF5A (IP:RMX00017, 4ug; Detection:RMX00017 1:4000) with U-87 MG cells lysate 1520 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb™ KIF5A Multi-Recombinant antibody (RMX00017)

    IHC staining of mouse brain using RMX00017

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00017 (KIF5A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Multi-rAb™ KIF5A Multi-Recombinant antibody (RMX00017)

    IHC staining of mouse brain using RMX00017

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00017 (KIF5A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Multi-rAb™ KIF5A Multi-Recombinant antibody (RMX00017)

    IF Staining of PC-12 using RMX00017

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (RMX00017) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Multi-rAb™ KIF5A Multi-Recombinant antibody

KIF5A Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00017

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050, Neuronal kinesin heavy chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, 87-MG cells, mouse brain tissue, rat brain tissue
Positive IP detected inU-87 MG cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00017 targets KIF5A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 118-120 kDa
GenBank accession numberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
UNIPROT IDQ12840
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Protocols

Product Specific Protocols
WB protocol for KIF5A antibody RMX00017Download protocol
IHC protocol for KIF5A antibody RMX00017Download protocol
IF protocol for KIF5A antibody RMX00017Download protocol
IP protocol for KIF5A antibody RMX00017Download protocol
Standard Protocols
Click here to view our Standard Protocols