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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using KIF5A Recombinant antibody (84013-4-RR)

    WB analysis of rat brain using 84013-4-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF5A Recombinant antibody (84013-4-RR)

    IHC staining of mouse brain using 84013-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84013-4-RR (KIF5A antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using KIF5A Recombinant antibody (84013-4-RR)

    IF Staining of PC-12 using 84013-4-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K-562 cells using KIF5A Recombinant antibody (84013-4-RR)

    FC experiment of K-562 using 84013-4-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using KIF5A Recombinant antibody (84013-4-RR)

    FC experiment of SH-SY5Y using 84013-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84013-4-RR

    Affinity and Kinetic Characterization of 84013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

KIF5A Recombinant monoclonal antibody

KIF5A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84013-4-RR
Clone No.241124H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat brain tissue, U-87-MG cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inK-562 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84013-4-RR targets KIF5A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15518

Product name: Recombinant human KIF5A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 931-1032 aa of BC146670

Sequence: SPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS

相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 118-120 kDa
GenBank accession numberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
RRIDAB_3671581
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ12840
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Protocols

Product Specific Protocols
FC protocol for KIF5A antibody 84013-4-RRDownload protocol
IF protocol for KIF5A antibody 84013-4-RRDownload protocol
IHC protocol for KIF5A antibody 84013-4-RRDownload protocol
WB protocol for KIF5A antibody 84013-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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