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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIF4A Polyclonal antibody (14344-1-AP)

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIF4A Polyclonal antibody (14344-1-AP)

    IF Staining of HepG2 using 14344-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF4A antibody 14344-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

KIF4A Polyclonal antibody

KIF4A Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14344-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

Chromokinesin A, Chromokinesin-A, Chromosome-associated kinesin KIF4A, HSA271784, KIF4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14344-1-AP targets KIF4A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5682

Product name: Recombinant human KIF4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC050548

Sequence: MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAEL

相同性解析による交差性が予測される生物種
Full Name kinesin family member 4A
Calculated molecular weight 140 kDa
GenBank accession numberBC050548
Gene Symbol KIF4A
Gene ID (NCBI) 24137
RRIDAB_2132200
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for KIF4A antibody 14344-1-APDownload protocol
IHC protocol for KIF4A antibody 14344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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