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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIF2A Polyclonal antibody (13105-1-AP)

    WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)

    WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KIF2A Polyclonal antibody (13105-1-AP)

    WB analysis using 13105-1-AP (same clone as 13105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)

    IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KIF2A Polyclonal antibody (13105-1-AP)

    IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using KIF2A Polyclonal antibody (13105-1-AP)

    IF Staining of MDCK using 13105-1-AP (same clone as 13105-1-PBS)

    Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using KIF2A Polyclonal antibody (13105-1-AP)

    FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF2A Polyclonal antibody, PBS Only

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13105-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13105-1-PBS targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2A fusion protein Ag3748 相同性解析による交差性が予測される生物種
Full Name kinesin heavy chain member 2A
Calculated molecular weight 679 aa, 77 kDa
Observed molecular weight 80-100 kDa
GenBank accession numberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). High expression of KIF2A correlates with advanced tumor stages and worse prognosis in non-small cell lung cancer patients (PMID: 31858647). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).