Validation Data Gallery
Tested Applications
| Positive WB detected in | MDCK cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 9 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
Product Information
17422-1-AP targets KIF26B in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11446 Product name: Recombinant human KIF26B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-355 aa of BC042481 Sequence: AFSAVIHDKLQVPNTIRKAWNDRDNRCDICATHLNQLKQEAIQMVLTLEQAAGSEHYDASPCSPPPLSNIPTLVGSRHVGGLQQPRDWAFVPAPCATSNYTGFANKHGSKPSSLGVSNGAEKKSGSPTHQAKVSLQMATSPSNGNILNSVAIQAHQYLDGTWSLSRTNGVTLYPYQDSEALVTRGRGRLEDSAAAPALMSEDRGA 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 26B |
| Calculated molecular weight | 2108aa,224 kDa; 162aa,17 kDa |
| Observed molecular weight | 224-230 kDa |
| GenBank accession number | BC042481 |
| Gene Symbol | KIF26B |
| Gene ID (NCBI) | 55083 |
| RRID | AB_2131762 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2KJY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF26B antibody 17422-1-AP | Download protocol |
| WB protocol for KIF26B antibody 17422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
| ||
Biomed Pharmacother KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer. | ||
J Bone Miner Res KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.
| ||
J Ginseng Res Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop | ||
Cell Cycle Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer | ||
Biochem Biophys Res Commun Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.
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