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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF21A Polyclonal antibody (27276-1-AP)

    WB analysis using 27276-1-AP (same clone as 27276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using KIF21A Polyclonal antibody (27276-1-AP)

    WB analysis of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF21A Polyclonal antibody (27276-1-AP)

    IP experiment of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    IP result of anti-KIF21A (IP:27276-1-AP, 4ug; Detection:27276-1-AP 1:500) with mouse brain tissue lysate 5600ug. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIF21A Polyclonal antibody (27276-1-AP)

    IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIF21A Polyclonal antibody (27276-1-AP)

    IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

KIF21A Polyclonal antibody, PBS Only

KIF21A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27276-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27276-1-PBS targets KIF21A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26192

Product name: Recombinant human KIF21A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1125-1230 aa of NM_001173463

Sequence: MPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSTGDASLPGPLTPVAEGQEIGMNTETSGTSAREKELSPPPGLPSKIGSISRQSSL

相同性解析による交差性が予測される生物種
Full Name kinesin family member 21A
Calculated molecular weight 187 kDa
Observed molecular weight 190-200 kDa
GenBank accession numberNM_001173463
Gene Symbol KIF21A
Gene ID (NCBI) 55605
RRIDAB_2880825
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4S6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIF21A is well known for its association with congenital fibrosis of the extraocular muscles type 1 (CFEOM1). CFEOM1-related mutations in KIF21A relieve autoinhibition of the motor and third coiled-coil stalk, which results in enhanced accumulation of KIF21A in axonal growth cones and aberrant axon morphology in the oculomotor nerve.(PMID: 38767486)

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