Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
15263-1-AP targets KIF1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7263 Product name: Recombinant human KIF1B protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 230-579 aa of BC001415 Sequence: TSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRERLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCLQLLTHTFNREFSQVHGSVSDCKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSRASSPCPEFEQFQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDMNDWLYAFNPLLAGTIRSKLSRRCPSQSKY 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 1B |
| Calculated molecular weight | 204 kDa |
| Observed molecular weight | 199 kDa, 204 kDa |
| GenBank accession number | BC001415 |
| Gene Symbol | KIF1B |
| Gene ID (NCBI) | 23095 |
| RRID | AB_2131412 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60333 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF1B antibody 15263-1-AP | Download protocol |
| IHC protocol for KIF1B antibody 15263-1-AP | Download protocol |
| IP protocol for KIF1B antibody 15263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
Exp Neurol Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI | ||
Cell Rep Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.
| ||
Cell Death Dis AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo. | ||