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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF1A Polyclonal antibody (24841-1-AP)

    WB analysis using 24841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24841-1-AP (KIF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KIF1A Polyclonal antibody (24841-1-AP)

    IF Staining of SH-SY5Y using 24841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF1A antibody (24841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

KIF1A Polyclonal antibody

KIF1A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24841-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ATSV, C2orf20, HUNC 104, HUNC104, hUnc-104

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24841-1-AP targets KIF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18817

Product name: Recombinant human KIF1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1508-1566 aa of BC111799

Sequence: CPSLVEGRYGATDLRTPQPCSRPASPEPELLPEADSKKLPSPARATETDKEPQRLLVPD

相同性解析による交差性が予測される生物種
Full Name kinesin family member 1A
Calculated molecular weight 1690 aa, 191 kDa
Observed molecular weight191-203 kDa
GenBank accession numberBC111799
Gene Symbol KIF1A
Gene ID (NCBI) 547
RRIDAB_3669455
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for KIF1A antibody 24841-1-APDownload protocol
WB protocol for KIF1A antibody 24841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Feedback loops between DNMT1 and autophagy as well as senescence promotes organ aging and canities.

Authors - Lu Li
View Article
mouseWB,IF

J Headache Pain

CREB-KIF1A-CGRP-positive feedback loop drives central sensitization in chronic migraine.

Authors - Wei Jiang
  • KD Validated
View Article
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