Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85046-1-RR targets KIF17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6217 Product name: Recombinant human KIF17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC065927 Sequence: MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYLEIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRSHSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDGRCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALLR 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 17 |
| Calculated molecular weight | 115 kDa |
| Observed molecular weight | 170 kDa |
| GenBank accession number | BC065927 |
| Gene Symbol | KIF17 |
| Gene ID (NCBI) | 57576 |
| RRID | AB_3743967 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9P2E2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF17 antibody 85046-1-RR | Download protocol |
| IHC protocol for KIF17 antibody 85046-1-RR | Download protocol |
| WB protocol for KIF17 antibody 85046-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |