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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF15 Recombinant antibody (82903-1-RR)

    WB analysis using 82903-1-RR (same clone as 82903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KIF15 Recombinant antibody (82903-1-RR)

    WB analysis of HeLa using 82903-1-RR (same clone as 82903-1-PBS)

    WB result of KIF15 antibody (82903-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KIF15 Recombinant antibody (82903-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR (same clone as 82903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82903-1-RR

    Affinity and Kinetic Characterization of 82903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

  • Featured Product
  • KD/KO Validated

KIF15 Recombinant monoclonal antibody, PBS Only

KIF15 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82903-1-PBS
Clone No.230049F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82903-1-PBS targets KIF15 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33897

Product name: Recombinant human KIF15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 950-1050 aa of NM_020242

Sequence: EQQMAKVQKLEESLLATEKVISSLEKSRDSDKKVVADLMNQIQELRTSVCEKTETIDTLKQELKDINCKYNSALVDREESRVLIKKQEVDILDLKETLRLR

相同性解析による交差性が予測される生物種
Full Name kinesin family member 15
Calculated molecular weight160 kDa
Observed molecular weight160 kDa
GenBank accession numberNM_020242
Gene Symbol KIF15
Gene ID (NCBI) 56992
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NS87
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

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